USA USA: 1-631-626-9181 Europe Europe: 44-207-097-1828  
Search

Tissue samples, tissue arrays, cells, microorganisms, probes and services for your research.

Web Search    Google
Featured Products
Ordering Information

Payment methods we support:
✭ Invoice / Purchase Order
✭ Credit card


QualiCell® Induced Pluripotent Stem Cell-XLC366

  • Specification
  • Recommended Products
Cat.No.
CSC-C00115
Description
SEVERE COMBINED IMMUNODEFICIENCY, AUTOSOMAL RECESSIVE, T CELL-NEGATIVE, B CELL-NEGATIVE, NK CELL-NEGATIVE, DUE TO ADENOSINE DEAMINASE DEFICIENCY; Induced pluripotent stem cell line derived from fibroblast by reprogramming with lentiviral constructs encoding OCT4 (also known as POU5F1), SOX2, Klf4 and cMyc. ADA mutations found in fibroblasts used for reprogramming were verified in the iPSCs -- cells were compound heterozygous for mutations in the ADA gene: one allele had a G>A transition at nucleotide 646 in exon 7 of the ADA gene [646G>A] resulting in a substitution of arginine for glycine at codon 216[Gly216Arg(G216R)]; the other allele had a 5-nucleotide deletion (del nt1050-54; GAAGA), found by direct sequence analysis of exon 10 (PMID: (Park et al. Cell 134:877-86, 2008).
Organism
Human
Karyotype
46,XY.arr(1-22)x2,(XY)x1
Source
Heritable Diseases; Gender: Male Age: 3 MO Race: Caucasian Biopsy Source: Skin
Culture Properties
Feeder Dependent
Recommended Medium
DMEM/F-12 Medium
Quality Control
The cell line submitted to the Repository frozen was recovered and expanded. The expanded line was evaluated for viability surface antigen expression and alkaline phosphatase activity. Pluripotency was assessed via embryoid body (EB) formation and directe
Storage and Shipping
Liquid nitrogen. Dry ice.
Citation Guidance
If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the Pub Med ID of your paper to get a coupon.
Customer Support & Price Inquiry
Please input "bioarray" as verification code.
CONTACT US USA
45-1 Ramsey Road, Shirley, NY 11967, USA
Tel: 1-631-626-9181
Fax: 1-631-614-7828
Email: 

Europe
Tel: 44-207-097-1828
Email: