The PC-12 cell line was derived from a transplantable rat pheochromocytoma. The cells respond reversibly to NGF by induction of a neuronal phenotype. The cells do not synthesize epinephrine. PC-12 adheres poorly to plastic and tends to grow in small clusters. Attachment is improved by using collagen coated flasks. The species was confirmed by Real-Time PCR.
Rattus norvegicus, rat
floating clusters; few scattered lightly attached cells.
small irregularly shaped cells
40 chromosomes; 38 autosomes plus XY
This cell line is a suitable transfection host.
Tests for mycoplasma, bacteria and fungi were negative
Storage and Shipping
liquid nitrogen vapor phase
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