Established from the mastocytoma tumor of a DBA/2 mouse treated with methylcholanthrene. P-815 cells phagocytose latex beads but not zymosan or BCG. They do not function in antibody dependent cell mediated cytotoxicity. Growth of the cells is not inhibited by dextran sulfate, LPS or PPD. Tested and found negative for ectromelia virus (mousepox).
Mus musculus (mouse)
Murine near-diploid karyotype-41(36-43)-1-2 centric fusion markers present.
Mycoplasma specific PCR: negative; Bacteria specific PCR: negative
Storage and Shipping
Frozen with 70% medium, 20% FBS, 10% DMSO at about 5 x 10^6 cells/ampoule; ship in dry ice; store in liquid nitrogen
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