This cell line is established from Mouse Swiss NIH embryo.These cells are sensitive to sarcoma virus focus formation and leukemia virus propogation. Cells have now lost their contact inhibition. These cells are useful for DNA transfection and transformation studies; tested and found negative for ectromelia virus (mousepox). Fetal calf serum is no suitable. The serum initially employed and found to be satisfactory was from the Colorado Serum Company.
STR DNA Profile
Fluorescence (DAPI) test: negative; Mycoplasma specific PCR: negative; Bacteriamspecific PCR: negative
Storage and Shipping
liquid nitrogen vapor phase
This cell line is a suitable transfection host.
The NIH/3T3 cell line is highly sensitive to sarcoma virus focus formation and leukemia virus propagation and has proven to be very useful in DNA transfection studies.
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