Species: mouse-male, neonatal
It is characteristic of this line to shed viable cells into the medium, thus rendering the medium turbid and giving the gross impression of bacterial contamination. The shed cells are viable and may be used to initiate new cultures.
Mus musculus ,mouse
, adjusted to contain 1.5 g/L sodium bicarbonate and 10% horse serum
Tests for mycoplasma, bacteria and fungi were negative
Storage and Shipping
Frozen with 52.5% RPMI-1640, 40% FBS, 7.5% DMSO at about 4-5 x 10^6 cells/ampoule; ship in dry ice; store in liquid nitrogen
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