This line is a gastric carcinoma cell line derived in 1976 and associates at the National Cancer Institute from a liver metastasis of a well differentiated carcinoma of the stomach taken prior to cytotoxic therapy. The tumor was passaged as a xenograft in athymic nude mice for three passages before the cell line was established. The cells are L-dopa decarboxylase(DDC) negative. This lines were minimally positive for vasoactive intestinal peptide(VIP) receptors and lacked gastrin receptors. They expressed receptors for muscarinic cholinergic agents. No evidence of amplification or rearrangements was noted with the N-myc, L-myc, myb and EGF receptor genes. The cell line expressed levels of C-myc and c-erb-B2 RNA that were comparable to other cell lines. There was no expression of the following genes: N-myc, L-myc, c-cis, IGF-2, or gastrin releasing peptide. This lines have a reported plating efficiency of 4.3%. They grow as an adherent monolayer of tightly knit epithelial cells.
STR DNA Profile
near diploid; DM were present in 64% of cells examined
Tests for mycoplasma, bacteria and fungi were negative
Storage and Shipping
Frozen with 52.5% RPMI-1640, 40% FBS, 7.5% DMSO at about 4-5 x 10^6 cells/ampoule
Shipping Condition: Room Temperature
If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the Pub Med ID of your paper to get a coupon.