Species: human - male, 33 years old, Caucasian
Tumorigenecity: yes, in nude mice
Isoenzyme: G6PD, B;PGM1, 1;PGM3, 1-2;ES-D, 1;AK-1, 1;Me-2, 1;GLO-1, 1-2
Note: This line was derived from cells present in ascites fluid obtained from a patient after treatment with 5-fluorouracil. The cells contain Dopa decarboxylase and, unlike other colorectal lines, contain cytoplasmic dense core granules characteristic of endocrine secretion. The cells do not express the TAG-72 or CA-19-9 antigens nor do they produce carcinoembryonic antigen.
STR DNA Profile
no Y chromosome was detected ; a large number of DM
colon; colorectal; cecal
floating aggregates of round cells with some attached cells.
Tests for mycoplasma, bacteria and fungi were negative
Storage and Shipping
Frozen with 52.5% RPMI-1640, 40% FBS, 7.5% DMSO at about 4-5 x 10^6 cells/ampoule
Shipping Condition: Room Temperature
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