NCI-H69/LX4 has been established by exposure of the parent line, H69 to doxorubicin, also known as adriamycin. The line hyperexpresses P-glycoprotein and demonstrates a multidrug resistant drug accumulation deficit. It is recommended to culture the cells without drug after resuscitation until the first passage.
Aggregates in suspension
RPMI + 2mM Glutamine + 0.4µg/ml doxorubicine + 10% Foetal Bovine Serum (FBS).
Creative Bioarray ships frozen cells on dry ice. On receipt, immediately transfer frozen cells to liquid nitrogen (-180 °C) until ready for experimental use.
Never can cryopreserved cells be kept at -20 °C.
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