The drug-resistant cell line NCI-H69/LX20 has been derived by exposure of the parent line, H69 to doxorubicin, also known as adriamycin. These multi drug resistant (MDR) cells have been found to be recognised by various anti-P-glycoprotein antibodies.
Aggregates in suspension
RPMI 1640 + 2mM Glutamine + 2µg/ml Doxorubicin + 10% Foetal Bovine Serum (FBS). The following supplement will assist good growth of cells: sodium selenite 10nM, Insulin 250ng/ml, Transferrin 10ug/ml and sodium pyruvate 1mM.
Creative Bioarray ships frozen cells on dry ice. On receipt, immediately transfer frozen cells to liquid nitrogen (-180 °C) until ready for experimental use.
Never can cryopreserved cells be kept at -20 °C.
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