NCI-H69/CPR is a drug resistant subline of NCI-H69. Drug resistance was developed by addition of a stepwise increase in cisplatin to the growth medium of the parental line. The cell line exhibits a 5-fold resistance to cisplatin and is cross resistant to melphalan. A significant change in cellular glutathione content or sensitivity to cadmium chloride (as indicator of metallothionein content) was not detected, but changes in glutathione-S-transferase activity were seen. Cisplatin accumulation was unchanged compared with the parent line. It is recommended to culture the cells without drugs after resuscitation until the first passage.
Aggregates in suspension
RPMI 1640 + 2mM Glutamine + 0.4µg/ml cisplatin + 10% Foetal Bovine Serum (FBS).
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