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NCI-H661

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Cat.No.
CSC-C8224L
Description
Established from a 43-year-old Caucasian male. The line lacks ultrastructural and biochemical evidence of squamous differentiation or mucin production. The cells express easily detectable p53 mRNA at levels comparable to normal lung tissue, and exhibit no gross structural DNA abnormalities. The cells stain positively for keratin and vimentin but are negative for neurofilament triplet protein.
Tissue of Origin
lung; derived from metastatic site: lymph node
Organism
Homo sapiens, human
STR DNA Profile
Amelogenin: X,Y
CSF1PO: 10
D13S317: 11
D16S539: 12
D5S818: 11
D7S820: 8,10
THO1: 8
TPOX: 8
vWA: 17
Karyotype
hyperhexaploid; modal number = 142; range = 130 to 153. Each cell had over 70 marker chromosomes. Among the markers were t(1p10q), del(1)(q11/q12), 6q+, 2q+,der(20)t(12;20)(q11;q13). Some of these had 2 to copies per cell. Most cells also had 5 to 10 microchromosomes (metacentric like). Structurally normal N2,N4 and N9 were absent; two copies of the X and multiple copies of the Y were detected.
Source
Lung
Culture Properties
adherent
Morphology
epithelial
Recommended Medium
90% RPMI1640 + 10% h.i. FBS
Quality Control
Tests for mycoplasma, bacteria and fungi were negative
Storage and Shipping
liquid nitrogen vapor phase
Disease
carcinoma; large cell lung cancer
Citation Guidance
If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the Pub Med ID of your paper to get a coupon.
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