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NCI-H596

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Cat.No.
CSC-C9538L
Description
Species: human - male, 73 years old, Caucasian
Abnormal genes: p53 : LOH(+)
Tumorigenecity: yes, in nude mice
Isoenzyme: G6PD, B; PGM1, 1;PGM3, 1;ES-D, 1;Me-2, 0;AK-1, 1;GLO-1, 1;Phenotype Frequency Product = 0.0362
Histopathology: carcinoma, adenosquamous; stage 3A
Note: This line was derived from a tumor mass in the chest wall of a patient with adenosquamous carcinoma of the lung. The specimen was obtained prior to therapy. The cells express easily detectable levels of p53 mRNA (comparable to levels found in normal lung), and exhibit no gross structural DNA abnormalities. The cells stain positively for keratin and vimentin, but are negative for neurofilament triplet protein. The cells express multiple markers of squamous differentiation, including cross linked envelope formation, transglutaminase activity and production of involucrin.
p53 mutation: GGC to TGC, ex7, 245 codon,
Kras codon 12: GGT
Organism
Human
STR DNA Profile
D3S1358: 14
vWA: 16
FGA: 23,25
Amelogenin: X
TH01: 7,9.3
TPOX: 8
CSF1P0: 13
D5S818: 11,13
D13S317: 11
D7S820: 11
Karyotype
This is a near triploid human cell line.
Source
lung, soft tissue
Culture Properties
monolayer
Morphology
epithelial
Quality Control
Tests for mycoplasma, bacteria and fungi were negative
Storage and Shipping
Frozen with 52.5% RPMI-1640, 40% FBS, 7.5% DMSO at about 4-5 x 10^6 cells/ampoule
Shipping Condition: Room Temperature
Citation Guidance
If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the Pub Med ID of your paper to get a coupon.
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