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NCI-H548

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Cat.No.
CSC-C9537L
Description
Species: human - male, 52 years old, Caucasian
Tumorigenecity: yes, in nude mice
Isoenzyme: G6PD, B; PGM1, 1 ;PGM3, 1;ES-D, 1;AK-1, 1-2;GLO-1, 2
Production: carcinoembryonic antigen, 18ng/ml per 10 exp6 cells per 10 days
Histopathology: adenocarcinoma
Note: This is one of 14 colorectal carcinoma cell lines derived by J.G. Park and associates during the years 1982 through 1985. The line was obtained from a well differentiated sigmoid tumor from a patient prior to therapy. The line was initially grown in medium with fetal bovine serum, but was later adapted to growth in the chemically defined medium ACL-4. Floating aggregates produce tubuloglandular structures lined by columnar epithelia. When grown in medium with serum, the cell attach partially to the substrate and form domes. The cells and tumors formed from the cells are positive for Dopa decarboxylase and are negative for the TAG-72 antigen
Organism
Human
STR DNA Profile
D3S1358: 16
vWA: 15,18
FGA: 21,22
Amelogenin: X
TH01: 7
TPOX: 10,11
CSF1P0: 11
D5S818: 12,13
D13S317: 9,11
D7S820: 9,11
Karyotype
the Y chromosome is not present in most of the cells
Source
colon
Culture Properties
floating aggregates of round cells with some attached cells
Morphology
epithelial
Recommended Medium
ACL-4 medium with modifications
Quality Control
Tests for mycoplasma, bacteria and fungi were negative
Storage and Shipping
Frozen with 52.5% RPMI-1640, 40% FBS, 7.5% DMSO at about 4-5 x 10^6 cells/ampoule
Shipping Condition: Room Temperature
Citation Guidance
If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the Pub Med ID of your paper to get a coupon.
CSC-6317W
CSC-C0204
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CSC-C0256
CSC-C0300
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