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NCI-H295R

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Cat.No.
CSC-C8887H
Description
NCI-H295R was adapted from the NCI-H295 pluripotent adrenocortical carcinoma cell line established by A.F. Gazdar and associates (1990) from a carcinoma of the adrenal cortex. The original cells were adapted to a culture medium which decreased the population doubling time from 5 days to 2 days. While the original cells grew in suspension, the adapted cells were selected to grow in a monolayer. This cell line retains the ability to produce adrenal androgens. It is responsive to angiotensin II and potassium ions.
Organism
Homo sapiens ,human
STR DNA Profile
Amelogenin: X,X
CSF1PO:10,12
D13S317: 13
D16S539: 11
D5S818: 12
D7S820: 9,12
TH01: 9.3
TPOX: 8
vWA: 17,18
D3S1358: 15,16
D21S11: 32.2
D18S51: 17
Penta E: 5,12
Penta D: 8
D8S1179: 13
FGA: 19.2, 24
Source
adrenal gland
Morphology
epithelial
Recommended Medium
DMEM/Ham's F12 (1:1 mixture) containing 15 mM HEPES, 0.00625 mg/ml insulin, 0.00625 mg/ml transferrin, 6.25 ng/ml selenium, 1.25 mg/ml bovine serum albumin, and 0.00535 mg/ml linoleic acid and 2.5% Nu-Serum I.
Quality Control
Tests for mycoplasma, bacteria and fungi were negative
Storage and Shipping
liquid nitrogen vapor phase
Citation Guidance
If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the Pub Med ID of your paper to get a coupon.
CSC-6321W
CSC-C0316
CSC-C0351
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