The NCI-H146 cell line was derived by A.F. Gazdar and associates in 1979 from the pleural fluid of a patient with small cell cancer of the lung. The bone marrow specimen was taken prior to therapy. The line expresses elevated levels of four biochemical markers (neuron specific enolase, brain isoenzyme of creatine kinase, L-DOPA decarboxylase and bombesin-like immunoreactivity). The cells stain positively for vimentin and keratin, but are negative for neurofilament triplet protein. The cells produce relatively high amounts of c-myc mRNA, but c-myc DNA sequences are not amplified. The cells do not express vasopressin, oxytocin or gastrin releasing peptide.
Homo sapiens ,human
STR DNA Profile
Penta E: 12
Penta D: 13
This is a near triploid human cell line. The modal chromosome number is 68, but cells with 66, 70 and 71 chromosomes also occurred frequently. At least 15 markers were common to all cells including single copies of der(1)t(1;13)(p32;q12) and del(3)(p21/22), and paired der(7)t(7;?)(p22;?), t(13q16q) and t(16p21q). Ten others were seen in single copies or paired. No HSR or DM were found. Structurally normal N5, N13 and N14 were absent. Four or more copies of N18 and N20 were present in each cell, and N5, N7 and N11 were mostly single copies per cell. The X chromosomes were paired, and no Y chromosome was detected in QM stained preparations.
Lung (pleural effusion)
Tests for mycoplasma, bacteria and fungi were negative
Storage and Shipping
liquid nitrogen vapor phase
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