Mesenchymal stem cells are harvested and cultured from normal human bone marrow. Cell purity is far higher than cells from traditional Dexter cultures. Cells are tested for purity by flow cytometry and for their ability to differentiate into osteogenic, chondrogenic and adipogenic lineages. Cells are positive for CD105, CD166, CD29, and CD44. Cells test negative for CD14, CD34 and CD45. Media systems are available to support growth of hMSCs and their differentiation into adipogenic, chondrogenic, and osteogenic lineages.
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