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LS513;LS 513

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Cat.No.
CSC-C9483
Description
LS513 is a colorectal carcinoma cell line isolated in 1985 from a primary tumor biopsy from a 63 year old Caucasian male patient diagnosed with a Dukes C mucin secreting cecal tumor located at the Bauhin valve. Approximately 50% of LS513 cells express surface carcinoembryonic antigen (CEA). LS513 cells express the major histocompatibility (MHC) class I antigens HLA and beta 2 microglobulin. MHC class II antigens (HLA-DR, DQ, and DP were not detected). TGF beta-1 is inhibitory for proliferation of LS513 cells, whereas TGF beta-2 has no effect on the growth of these cells. LS513 cells are 100-fold less sensitive to TGF beta-1 than the LS-1034 cell line. LS513 cells are multidrug resistant (MDR) and are tumorigenic in nude mice. Colony forming efficiency was 30% in methylcellulose.
Tissue of Origin
Cecum
Organism
Homo sapiens (human)
Recommended Medium
Culture Properties
monolayer
Morphology
epithelial
STR DNA Profile
Amelogenin: X,Y
CSF1PO: 10,10
D13S317: 9,10
D16S539: 12,13
D5S818: 11,11
D7S820: 8,11
THO1: 8
TPOX: 8
vWA: 16,17
D3S1358: 15
D21S11: 30
D18S51: 12,18
Penta E: 5,18
Penta D: 9,14
D8S1179: 13
FGA: 19,21
Karyotype
Two stem lines could be distinguished. The main one was represented in 65% of the cells, with a modal number of 51,XY and 3 markers, M1 - der(1)t(1;15), M2 - der(2)t(2;3)der(3)t(2;3), M3, and a monosomy 15. The second stem line had a modal chromosome number of 52,XY and presented M2 and M3 plus an isochromosome for the long arm of chromosome 1 called M4. A trisomy 5,7, a tetrasomy 13, and a monosomy 2 and 3 were present in all of the cells analyzed; the line did not exhibit monosomy 15.
Quality Control
Tests for mycoplasma, bacteria and fungi were negative
Storage and Shipping
Frozen with 52.5% RPMI-1640, 40% FBS, 7.5% DMSO at about 4-5 x 10^6 cells/ampoule; ship in dry ice; store in liquid nitrogen
Citation Guidance
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