The cells are positive for Colon Antigen 3. They are negative for p53 antigen expression, but positive for mRNA expression. The line was derived from the same tumor as LS 180. LS 174T cells stain positively for cytokeratins.
Homo sapiens (human)
STR DNA Profile
Penta E: 15,16
Penta D: 10
45,X; one X chromosome missing; no other chromosomal aberrations
Tests for mycoplasma, bacteria and fungi were negative
Storage and Shipping
Frozen with 52.5% RPMI-1640, 40% FBS, 7.5% DMSO at about 4-5 x 10^6 cells/ampoule; ship in dry ice; store in liquid nitrogen
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