Established from the left supraclavicular lymph node metastasis from a 50-year-old man with prostate carcinoma in 1977; cells were described to be androgen-sensitive
Human hypotetraploid karyotype with 6% polyploidy - 88(84-93)<4n>XXYY, -2, -4, -6, -6, -13, -13, +2mar, t(1;15)(p22.3;q22.3)x2, del(2)(p16p23), del(10)(q24)x2, der(16)t(6;16)(p22.1;q22)x2 - del(10)(q24) is recurrent in prostate carcinoma - corresponds to
adherent fibroblastoid cells growing in aggregates and as single cells
Mycoplasma: negative in DAPI, microbiological culture, RNA hybridization, PCR assays
Immunology: cytokeratin +, cytokeratin-7 -, cytokeratin-8 +, cytokeratin-17 -, cytokeratin-18 +, cytokeratin-19 +, desmin -, endothel -, EpCAM +, GFAP -, neurofilament -,
Storage and Shipping
Frozen with 70% medium, 20% FBS, 10% DMSO at about 3 x 10^6 cells/ampoule; ship in dry ice; store in liquid nitrogen
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