The cell line LMH was established in 1981 by Tomoyuki Kitagawa (see reference below) at the Cancer Institute, Kami-Ikebukuro-ku of Tokyo, Japan. Primary hepatocellular carcinoma epithelial cell lines were transformed into permanent cells by inducing tumorous nodules by long term treatment with diethylnitrosamine in the liver of a male leghorn cchicken. LMH cells are inducible for the expression of the liver specific apolipoprotein II (apoll) gene. The species was confirmed by Real-Time PCR.
Gallus gallus ,chicken
Triploid; modal number = 116; six marker chromosomes.
Waymouth's MB 752 medium supplemented with L-glutamine and 10% fetal bovine serum.
Tests for mycoplasma, bacteria and fungi were negative
Storage and Shipping
liquid nitrogen vapor phase
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