Established from the bone marrow metastasis of a 0.4-year-old boy with neuroblastoma
polymorph adherent cells growing in monolayers and in small clumps
Mycoplasma: negative in microbiological culture, PCR assays
Immunology: cytokeratin -, desmin -, endothel -, GFAP -, neurofilament +, vimentin +
Viruses: PCR: EBV -, HBV -, HCV -, HIV -, HTLV-I/-II -
Storage and Shipping
Frozen with 70% medium, 20% FBS, 10% DMSO at about 1 x 10^6 cells/ampoule; ship in dry ice; store in liquid nitrogen
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