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L1210;L-1210

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Cat.No.
CSC-C1071
Description
Established from the methylcholanthrene-induced tumor in a DBA strain mouse (subline 212, 8-month-old female) in 1949; the cells were propagated by heterotransplantation into DBA/2 mice; an in-vitro suspension culture was first reported in 1966; cells are used for cytotoxicity studies. Tested and found negative for ectromelia virus (mousepox)
Organism
mouse - female, 8 months old
Karyotype
Murine hypodiploid karyotype - 39(36-42), with about 75% polyploidy - about 77% of cells carry one or more rearrangements/aberrations.
Source
lymphocytic leukemia
Culture Properties
suspension
Morphology
lymphoblast
Recommended Medium
DMEM , 90%; horse serum., 10% - OR - Fischer's medium, 90%; horse serum., 10%.
Quality Control
Mycoplasma: negative in DAPI, microbiological culture, RNA hybridization, PCR assays
Viruses: ELISA: reverse transcriptase positive; PCR: SMRV -
Storage and Shipping
Frozen with 70% medium, 20% horse serum, 10% DMSO at about 5 x 10^6 cells/ampoule; ship in dry ice; store in liquid nitrogen
Citation Guidance
If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the Pub Med ID of your paper to get a coupon.
CSC-6256W
CSC-6264W
CSC-6284W
CSC-6285W
CSC-6286W
CSC-6287W
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