Established from the methylcholanthrene-induced tumor in a DBA strain mouse (subline 212, 8-month-old female) in 1949; the cells were propagated by heterotransplantation into DBA/2 mice; an in-vitro suspension culture was first reported in 1966; cells are used for cytotoxicity studies. Tested and found negative for ectromelia virus (mousepox)
mouse - female, 8 months old
Murine hypodiploid karyotype - 39(36-42), with about 75% polyploidy - about 77% of cells carry one or more rearrangements/aberrations.
Mycoplasma: negative in DAPI, microbiological culture, RNA hybridization, PCR assays
Viruses: ELISA: reverse transcriptase positive; PCR: SMRV -
Storage and Shipping
Frozen with 70% medium, 20% horse serum, 10% DMSO at about 5 x 10^6 cells/ampoule; ship in dry ice; store in liquid nitrogen
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