Established from the normal subcutaneous areolar and adipose tissue of a male C3H/An mouse; used as target in TNF detection assays.
Mus musculus (mouse)
Murine hypertriploid karyotype-61-67, 12-16 centric fusion markers present.
Connective tissue; normal; subcutaneous; areolar and adipose
fibroblasts growing as monolayer
Fluorescence (DAPI) test: negative; Mycoplasma specific PCR: negative; Bacteria
specific PCR: negative
Storage and Shipping
Frozen with 70% medium, 20% FBS, 10% DMSO at about 2 x 10^6 cells/ampoule; ship in dry ice; store in liquid nitrogen
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