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KYSE-510

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Cat.No.
CSC-C0422
Description
Established from the well-differentiated eosphageal squamous cell carcinoma with moderate invasion of the adventitia resected from cervical esophagus of a 67-year-old Japanese woman after cisplatin and radiotherapy; described as carrying p53 mutation and amplification of MYC, HST1, CYCLIN D1, and to induce well-differentiated tumors in nude mice
Organism
Human
Karyotype
Human hypertriploid karyotype with 8% polyploidy - 73(66-73)<3n>XXX, -X, +1, +1, -2, -2, +3, -4, +5, +6, +7, -14, -14, +19, -21, -21, +22, +9mar, add(X)(p22.2), add(1)(q21), ider(1)(q10)t(1;8)(q21;q11), add(2)(q11), del(3)(q21), del(3)(p1?), add(5)(p14),
Source
esophageal squamous cell carcinoma
Culture Properties
monolayer
Morphology
epitheloid cells growing as monolayer with multilayer foci
Recommended Medium
90%RPMI1640 or 45%RPMI1640 + 45%Ham's F-12 + 10%h.i. FBS.
Quality Control
Mycoplasma: contamination was eliminated with BM-Cyclin (tiamulin & minocycline), then negative in DAPI, microbiological culture, RNA hybridization, PCR assays
Immunology: cytokeratin +, cytokeratin-7 +, cytokeratin-8 +, cytokeratin-17 +, cytokeratin-18
Storage and Shipping
Frozen with 70% medium, 20% FBS, 10% DMSO at about 8 x 10^6 cells/ampoule; ship in dry ice; store in liquid nitrogen
Citation Guidance
If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the Pub Med ID of your paper to get a coupon.
CSC-C0314
CSC-C0404
CSC-C0405
CSC-C0414
CSC-C0419
CSC-C0423
Customer Support & Price Inquiry
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