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JVM-3
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Cat.No.
CSC-C0202
Description
Established from the peripheral blood of a 73-year-old man with B-prolymphocytic leukemia (PLL) at diagnosis; cell line was established by EBV-transformation during treatment with phorbol ester TPA; cells express mRNA of the proto-oncogenes BCL2 and BCL3
Species
Human
Source
chronic B cell leukemia
Culture Properties
suspension
Morphology
lymphoblastoid cells growing in clumps in suspension
Karyotype
Human near tetraploid karyotype without clear mode - 87-93<4n>XXYY, +3, -7, -8, -10, +12, +12, -14, -19, -19, -20, -20 - appears to be derivate of original hyperdiploid line which exhibited trisomy 12
Quality Control
Mycoplasma: contamination was eliminated with Ciprobay (ciprofloxacin), then negative in DAPI, microbiological culture, RNA hybridization, PCR assays
Immunology: CD3 -, CD10 -, CD13 -, CD19 +, CD20 +, CD34 -, CD37 +, cyCD79a +, CD80 +, HLA-DR +, sm/cyIgG
Immunology: CD3 -, CD10 -, CD13 -, CD19 +, CD20 +, CD34 -, CD37 +, cyCD79a +, CD80 +, HLA-DR +, sm/cyIgG
Storage and Shipping
Frozen with 70% medium, 20% FBS, 10% DMSO at about 10 x 10^6 cells/ampoule; ship in dry ice; store in liquid nitrogen
Citation Guidance
If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the PubMed ID of your paper to get a coupon.
- Q & A
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- Product Information Sheet
Q:
What is the difference between cell viability and cytotoxicity?
A:
A cell viability assay measures the number (relative or absolute) of living cells in a sample. Viability is a function of cellular proliferation and death. Cytotoxicity assays measure the number (relative or absolute) of dead cells.
Average Rating: 5.0 | 1 Scientist has reviewed this product
Top-notch quality
The durability of cell products in various conditions has greatly assisted in my research. Kudos to the provider's services.
22 Oct 2022
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