Established from the peripheral blood of a 78-year-old woman with B-cell non-Hodgkin lymphoma (B-NHL), specifically mantle cell lymphoma, large variant in leukemic conversion
Human highly rearranged flat-moded hypertriploid karyotype - 70-78<3n>XXXX, +X, +1, +2, +2, +4, +6, +7, -8, +10, +11, -12, -12, +13, +14, +15, -17, +18, -19, -19, -22, +2-4mar, del(X)(p21)/add(X)(p11.2)x2, der(X)t(X;11)(q26-28;q13)t(11;?10)(q24;?p12), der
round single cells growing in suspension
Mycoplasma: contamination was eliminated with BM-Cyclin (tiamulin & minocycline), then negative in microbiological culture, PCR assays Immunology: CD3 -, CD10 -, CD13 -, CD19 +, CD20 +, CD34 -, CD37 +, CD138 -, HLA-DR +, sm/cyIgG -, sm/cyIgM +, sm/cykappa
Storage and Shipping
Frozen with 70% medium, 20% FBS, 10% DMSO at about 3 x 10^6 cells/ampoule; ship in dry ice; store in liquid nitrogen
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