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J774A.1;J-774A.1

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Cat.No.
CSC-C1074
Description
Established from a tumor in a female BALB/c mouse in 1968; cells were described to synthesize lysozyme and interleukin-1 and to have receptors for immunoglobulin and complement. Active in antibody dependent phagocytosis; growth is inhibited by extran sulfate, PPD and LPS; tested and found negative for ectromelia virus (mousepox).
Organism
mouse - female
Karyotype
Cytogenetics: murine hypotetraploid karyotype - 78(70-81) - in most cells a centric fragment was present - mouse chromosome morphology confirmed
Source
monocytes-macrophages
Morphology
macrophage
Recommended Medium
DMEM and 10% h.i.FBS.
Quality Control
Mycoplasma: negative in DAPI, microbiological culture, RNA hybridization, PCR assays
Viruses: ELISA: reverse transcriptase positive; PCR: SMRV -
Storage and Shipping
Frozen with 70% medium, 20% FBS, 10% DMSO at about 1 x 10^6 cells/ampoule; ship in dry ice; store in liquid nitrogen
Citation Guidance
If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the Pub Med ID of your paper to get a coupon.
CSC-6256W
CSC-6264W
CSC-6284W
CSC-6285W
CSC-6286W
CSC-6287W
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