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Human Osteoblasts (HOB)

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Human osteoblasts are a highly specialized cell type of mesenchymal origin involved in bone formation and remodeling. In vivo, they produce the osteoid, a extracellular matrix rich in collagen type I. In the course of their natural maturation process into osteocytes, called osteogenesis, they become embedded in the bone matrix and stop proliferating. Creative Bioarray Human Osteoblasts (HOB) are produced at Creative Bioarray's cell culture facility and are available from knee and hip femoral tissue.
Shortly after isolation, all Creative Bioarray Human Osteoblasts are cryopreserved at passage 2 (P2) using Creative Bioarray's proprietary, serum-free freezing medium, Cryo-SFM. Each cryo vial contains more than 500,000 viable cells after thawing.
Proliferating cell cultures are made from 500,000 cryopreserved cells that have been thawed and cultured for three days at Creative Bioarray.
Storage and Shipping
Store in liquid nitrogen and ship in dry ice.
Citation Guidance
If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the Pub Med ID of your paper to get a coupon.
We are aiming to seed them in 3 biomaterial constructs. Do their behaviour for mineralization decrease with increase passaging?

The differentiation potential of any primary cell declines with ongoing population doublings. To obtain optimal differentiation rates, experiments should be performed as early in culture as possible.

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