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Human Bone Marrow Mononuclear Cells are approximately 15 to 25 μm in diameter. Unfortunately I don’t have any internal protocols for injecting mice. 27G may lyse the cells. So you may need to use 25 G needle. Prior to injecting the mice, I would recommend checking cell viability after passing the cells through the gauge needle you intend to use. You may be able to find some protocols online though.