This cell line is a derivative of the BW7756 mouse hepatoma that arose in a C57/L mouse. The cells can be propagated in a serum free medium consisting of DMEM, 75%; Waymouth's MAB 87/3 medium, 25% plus 3 x10-8 M selenium. Tested and found negative for ectromelia virus (mousepox).
Mus musculus (mouse)
Murine hypotetraploid karyotype with centric fusion and 6% polyploidy - flat-moded, 53-70<4n>.
Fluorescence (DAPI) test: negative; Mycoplasma specific PCR: negative; Bacteria
specific PCR: negative
Storage and Shipping
Frozen with 70% medium, 20% FBS, 10% DMSO at about 2.5 x 10^6 cells/ampoule; ship in dry ice; store in liquid nitrogen
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