Established from an adenocarcinoma from a 44-year-old female. Tumorigenic in nude mice and has ability to grow in agar. They have human adrenergic alpha2A urokinase receptor (u-PAR), vitamin D (moderate expression) and urokinase receptor (u-PAR); The cell line can produce secretory component of IgA, carcinoembryonic antigen (CEA), transforming growth factor beta binding protein and mucin. Ultrastructural features include microvilli, microfilaments, large vacuolated mitochondria with dark granules, smooth and rough ER with free ribosomes, lipid droplets, few primary and many secondary lysosomes. There is a G -> A mutation in codon 273 of the p53 gene resulting in an Arg -> His substitution.
Homo sapiens, human
STR DNA Profile
Penta E: 14,16
Penta D: 11,13
The stemline chromosome number is hypertriploid with the 2S component occurring at 2.4%. Seventeen marker chromosomes are found in most metaphases, generally in single copy per chromosome. The marker designations are: M1p-(=t(3p-;?) with a deleted short arm), t(7q;?), t(10q;?), i(13q), 19q+a; M6, ?t(8q;9q-), ?Xp, M9, 6q+, t(13;?)a, t(13;?)b, 19q+b, M14, M15, 15p+, and Xq-. Chromosome 13 is nullisomic and chromosomes 8 and 14 are generally monosomic. No Y chromosome was detected by QM band analysis.
Tests for mycoplasma, bacteria and fungi were negative
Storage and Shipping
liquid nitrogen vapor phase
HT-29 can be used as a suitable transfection host.
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