USA USA: 1-631-626-9181 Europe Europe: 44-208-144-6005  

Search

Tissue samples, tissue arrays, cells, microorganisms, probes and services for your research.

Web Search    Google
Featured Products
Ordering Information

Payment methods we support:
✭ Invoice / Purchase Order
✭ Credit card


HT-29

  • Specification
  • Recommended Products
Cat.No.
CSC-C8201L
Description
Established from an adenocarcinoma from a 44-year-old female. Tumorigenic in nude mice and has ability to grow in agar. They have human adrenergic alpha2A urokinase receptor (u-PAR), vitamin D (moderate expression) and urokinase receptor (u-PAR); The cell line can produce secretory component of IgA, carcinoembryonic antigen (CEA), transforming growth factor beta binding protein and mucin. Ultrastructural features include microvilli, microfilaments, large vacuolated mitochondria with dark granules, smooth and rough ER with free ribosomes, lipid droplets, few primary and many secondary lysosomes. There is a G -> A mutation in codon 273 of the p53 gene resulting in an Arg -> His substitution.
Tissue of Origin
Colon
Organism
Homo sapiens (human)
Recommended Medium
Culture Properties
adherent
Morphology
epithelial
STR DNA Profile
Amelogenin: X,X
CSF1PO: 11,12
D13S317: 11,11
D16S539: 11,12
D5S818: 11,12
D7S820: 10
THO1: 6,9
TPOX: 8,9
vWA: 17,19
D3S1358: 15,17
D21S11: 29,30
D18S51: 13
Penta E: 14,16
Penta D: 11,13
D8S1179: 10
FGA: 20,22
Karyotype
The stemline chromosome number is hypertriploid with the 2S component occurring at 2.4%. Seventeen marker chromosomes are found in most metaphases, generally in single copy per chromosome. The marker designations are: M1p-(=t(3p-;?) with a deleted short arm), t(7q;?), t(10q;?), i(13q), 19q+a; M6, ?t(8q;9q-), ?Xp, M9, 6q+, t(13;?)a, t(13;?)b, 19q+b, M14, M15, 15p+, and Xq-. Chromosome 13 is nullisomic and chromosomes 8 and 14 are generally monosomic. No Y chromosome was detected by QM band analysis.
Disease
colorectal adenocarcinoma
Applications
HT-29 can be used as a suitable transfection host.
Quality Control
Tests for mycoplasma, bacteria and fungi were negative
Storage and Shipping
liquid nitrogen vapor phase
Citation Guidance
If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the Pub Med ID of your paper to get a coupon.

For research use only. Not for any other purpose.

Customer Support & Price Inquiry
Please input "bioarray" as verification code.
CONTACT US USA
45-1 Ramsey Road, Shirley, NY 11967, USA
Tel: 1-631-626-9181
Fax: 1-631-614-7828
Email: 

Europe
Tel: 44-208-144-6005
Email: