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HT-29

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Cat.No.
CSC-C8201L
Description
Established from an adenocarcinoma from a 44-year-old female. Tumorigenic in nude mice and has ability to grow in agar. They have human adrenergic alpha2A urokinase receptor (u-PAR), vitamin D (moderate expression) and urokinase receptor (u-PAR); The cell line can produce secretory component of IgA, carcinoembryonic antigen (CEA), transforming growth factor beta binding protein and mucin. Ultrastructural features include microvilli, microfilaments, large vacuolated mitochondria with dark granules, smooth and rough ER with free ribosomes, lipid droplets, few primary and many secondary lysosomes. There is a G -> A mutation in codon 273 of the p53 gene resulting in an Arg -> His substitution.
Tissue of Origin
Colon
Organism
Homo sapiens, human
STR DNA Profile
Amelogenin: X,X
CSF1PO: 11,12
D13S317: 11,11
D16S539: 11,12
D5S818: 11,12
D7S820: 10
THO1: 6,9
TPOX: 8,9
vWA: 17,19
D3S1358: 15,17
D21S11: 29,30
D18S51: 13
Penta E: 14,16
Penta D: 11,13
D8S1179: 10
FGA: 20,22
Karyotype
The stemline chromosome number is hypertriploid with the 2S component occurring at 2.4%. Seventeen marker chromosomes are found in most metaphases, generally in single copy per chromosome. The marker designations are: M1p-(=t(3p-;?) with a deleted short arm), t(7q;?), t(10q;?), i(13q), 19q+a; M6, ?t(8q;9q-), ?Xp, M9, 6q+, t(13;?)a, t(13;?)b, 19q+b, M14, M15, 15p+, and Xq-. Chromosome 13 is nullisomic and chromosomes 8 and 14 are generally monosomic. No Y chromosome was detected by QM band analysis.
Source
Colon
Culture Properties
adherent
Morphology
epithelial
Recommended Medium
Quality Control
Tests for mycoplasma, bacteria and fungi were negative
Storage and Shipping
liquid nitrogen vapor phase
Disease
colorectal adenocarcinoma
Application
HT-29 can be used as a suitable transfection host.
Citation Guidance
If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the Pub Med ID of your paper to get a coupon.
CSC-6317W
CSC-C0204
CSC-C0253
CSC-C0256
CSC-C0300
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