This cell line was derived from peripheral blood leukocytes obtained by leukopheresis of a 36-year-old Caucasian female with acute promyelocytic leukemia. HL-60 cells spontaneously differentiate. Differentiation can be stimulated by butyrate, hypoxanthine, phorbol myristic acid (PMA, TPA), dimethylsulfoxide (DMSO, 1% to 1.5%), actinomycin D, and retinoic acid. The cells exhibit phagocytic activity and responsiveness to chemotactic stimuli.
Homo sapiens ,human
STR DNA Profile
Penta E: 13/13
Penta D: 10/12
Negative for mycoplasma: DAPINegative for bacteria and fungi: Thioglycollate, CASO-Bouillon EP
Storage and Shipping
liquid nitrogen vapor phase
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