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HL-60

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Cat.No.
CSC-C8216L
Description
This cell line was derived from peripheral blood leukocytes obtained by leukopheresis of a 36-year-old Caucasian female with acute promyelocytic leukemia. HL-60 cells spontaneously differentiate. Differentiation can be stimulated by butyrate, hypoxanthine, phorbol myristic acid (PMA, TPA), dimethylsulfoxide (DMSO, 1% to 1.5%), actinomycin D, and retinoic acid. The cells exhibit phagocytic activity and responsiveness to chemotactic stimuli.
Tissue of Origin
Peripheral Blood
Organism
Homo sapiens (human)
Culture Properties
Suspension
Morphology
lymphoblast
STR DNA Profile
Amelogenin: X/X
CSF1PO: 13/14
D13S317: 8/11
D16S539: 11/11
D5S818: 12/12
D7S820: 11/12
THO1: 7/8
TPOX: 8/11
vWA: 16/16
D3S1358: 16/16
D21S11: 30/30
D18S51: 14/14
Penta E: 13/13
Penta D: 10/12
D8S1179: 13/13
FGA: 22/24
Karyotype
modal no. 46, pseudodiploid
Markers
CD3 -, CD4 +, CD13 +, CD14 -, CD15 +, CD19 -, CD33 +, CD34 -, HLA-DR -
Applications
a. Hodgkin lymphoma related research
b. Study of myeloid differentiation
c. Cell-Based assay
d. In vivo efficacy study
Quality Control
Mycoplasma: Fluorescence (DAPI) test: negative; Mycoplasma specific PCR: negative; Selective biochemical test: negative
Viruses: ELISA: reverse transcriptase negative; PCR: EBV +, HBV -, HCV -, HHV-8 -, HIV-1 -, HIV-2 -, HTLV-I/II -, MLV -, SMRV -
Storage and Shipping
Ship in dry ice.
Store in liquid nitrogen.
BioSafety Level
BSL–1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.,
Citation Guidance
If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the Pub Med ID of your paper to get a coupon.

For research use only. Not for any other purpose.

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CONTACT US USA
45-1 Ramsey Road, Shirley, NY 11967, USA
Tel: 1-631-626-9181
Fax: 1-631-614-7828
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Europe
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