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HELA-S3

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Cat.No.
CSC-C0306
Description
The cell line is a subclone of its parent HELA derived in 1955. This cell line can be adapted to grow in suspension; the cells are positive for keratin by immunoperoxidase staining. The HELA-S3 clone has been very useful in the clonal analysis of mammalian cell populations relating to chromosomal variation, cell nutrition, and plaque-forming ability.
Organism
Homo sapiens, human
STR DNA Profile
D3S1358: 15,18
vWA: 16,18
FGA: 18,21
Amelogenin: X
TH01: 7
TPOX: 8,12
CSF1P0: 9,10
D5S818: 11,12
D13S317: 13.3
D7S820: 8,12
Karyotype
Human flat-moded hypotriploid karyotype with 32% polyploidy - 68(53-68)<3n>XX, -X, +1, -2, -4, +5, +8, +12, -13, -14, -20, -21, -22, -22, +4mar - del(1)(p35), der(1)t(1;3) (p11;q11), der(3)t(3;5)(q11;q11), i(5p)x2, del(6)(q23), add(6)(q27), del(7)(q32).
Source
Cervix
Culture Properties
Monolayer
Morphology
epithelial
Recommended Medium
Quality Control
Mycoplasma: negative in DAPI, microbiological culture, RNA hybridization, PCR assays
Immunology: cytokeratin +, cytokeratin-7 -, cytokeratin-8 +, cytokeratin-17 +, cytokeratin-18 +, cytokeratin-19 -, desmin -, endothel -, EpCAM -, GFAP -, neurofilament -,
Storage and Shipping
Frozen with 70% medium, 20% FBS, 10% DMSO at about 1.5 x 10^6 cells/ampoule; ship in dry ice; store in liquid nitrogen
Application
The HELA-S3 cell line can be used for tumourigenicity, virus studies (poliovirus 1 and Ad 3) and clonal analysis of mammalian cell populations.
Citation Guidance
If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the Pub Med ID of your paper to get a coupon.
CSC-6260W
CSC-6261W
CSC-6262W
CSC-6263W
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