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HCC-827

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Cat.No.
CSC-C0571
Description
Established from the lung of a 39-year-old woman with non-small cell lung cancer of the adenocarcinoma type; matched EBV+ B-lymphoblastoid cell line (B-LCL) is available (HCC-827BL). This lung adenocarcinoma has an acquired mutation in the EGFR tyrosine kinase domain (E746 - A750 deletion).
Organism
Homo sapiens, human
STR DNA Profile
D3S1358: OL
vWA: 18
FGA: 22,24
Amelogenin: X
TH01: 6
TPOX: 8
CSF1P0: 11
D5S818: 12
D13S317: 9
D7S820: 11,12
Karyotype
human flat-moded hypotriploid karyotype with 6% polyploidy - 62(54-66)<3n>XXX/XXXX, -1, +7, +7, -9, -10, -15, -17, -18, -21, -22, +3 mar, +0-4 dmin, add(X)(p11), dup(2)(p1?p2?), add(3)(p11), add(6)(p21), add(7)(p12), add(7)(p22), add(7)(q21.2;HSR), add(21)(p11), der(21;22)(q10;q10), add(22)(p11) - 2 large submetacentric HSR markers present
Source
Lung
Culture Properties
monolayer
Morphology
Epithelial
Recommended Medium
80-85% RPMI-1640 + 15-20% h.i. FBS
Quality Control
Mycoplasma: negative in microbiological culture, PCR assays
Immunology: cytokeratin +, cytokeratin-7 +, cytokeratin-8 +, cytokeratin-17 -, cytokeratin-18 +, cytokeratin-19 +, desmin -, endothel -, EpCAM +, GFAP -, neurofilament -, vimentin +
Viruses: PCR
Storage and Shipping
Frozen with 70% medium, 20% FBS, 10% DMSO at about 1 x 10^6 cells/ampoule; ship in dry ice; store in liquid nitrogen
Disease
Lung adenocarcinoma
Citation Guidance
If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the Pub Med ID of your paper to get a coupon.
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