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HCC-366

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Cat.No.
CSC-C0503
Description
Established from the tumor of an 80-year-old woman with non-small cell lung carcinoma (subtype adeno-squamous carcinoma); cells were described to carry fractional allellic loss with loss of heterozygosity at various hot spots; matched EBV+ B-lymphoblastoid cell line (B-LCL) is available (HCC-366BL)
Organism
Human
Karyotype
Human flat-moded hyperdiploid karyotype with 12% polyploidy - 48-56<2n>XX, +1, +1, -2, +4, +7, +8, +11, -13, -15, -18, +7-9mar, add(X)(p11), add(1)(p22), add(1)(q11)x1-2, add(4)(q31)x1-2, del(8)(p12), del(8)(q13), add(10)(p13), add(14)(p11), add(17)(p12)
Source
non-small cell lung carcinoma
Culture Properties
monolayer
Morphology
adherent epitheloid cells growing as monolayer
Recommended Medium
Quality Control
Mycoplasma: negative in microbiological culture, PCR assays
Immunology: cytokeratin +, cytokeratin-7 +, cytokeratin-8 +, cytokeratin-17 -, cytokeratin-18 +, cytokeratin-19 +, desmin -, endothel -, EpCAM +, GFAP -, neurofilament -, vimentin -
Viruses: PCR
Storage and Shipping
Frozen with 70% medium, 20% FBS, 10% DMSO at about 1.5 x 10^6 cells/ampoule; ship in dry ice; store in liquid nitrogen
Citation Guidance
If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the Pub Med ID of your paper to get a coupon.
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