Established from a primary ductal carcinoma of the breast mammary gland from a 24-year-old Caucasian woman in 1995; cells were described to be homozygous for the BRCA1 mutation; a paired EBV+ B-lymphoblastoid cell line (B-LCL HCC-1937BL) is available
adherent epithelial cells growing as monolayer, about 5-10% are giant vacuolated cells
Mycoplasma: negative in microbiological culture, PCR assays
Immunology: cytokeratin +, cytokeratin-7 +, cytokeratin-8 +, cytokeratin-17 +, cytokeratin-18 +, cytokeratin-19 -, desmin -, endothel -, EpCAM +, GFAP -, neurofilament -, vimentin +
Storage and Shipping
Frozen with 70% medium, 20% FBS, 10% DMSO at about 2 x 10^6 cells/ampoule; ship in dry ice; store in liquid nitrogen
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