Established from a primary, invasive, ductal breast carcinoma (TNM stage IIa, tumor size 5, grade 3, without lymph node metastasis) from a 52-year-old white woman prior to therapy in 1994; a paired EBV+ B-lymphoblastoid cell line (B-LCL HCC-1143BL) is available; reported to express EGP2, CK19 buth neither estrogen nor progesterone receptors are expressed
Human highly rearranged flat-moded hypertriploid karyotype with 8% polyploidy - 74-78<3n>XXX, -2, +3, -4, -6, +7, +7, +7, -9, -10, -11, -11, -13, -15, -17, -19, +20, +14-20 mar, +2 dmin, add(X)(p11.3), add(X)(p22)add(X)(q11), add(X)(q25-27), del(1)(p22),
epitheloid cells growing as adherent monolayers
Mycoplasma: negative in microbiological culture, PCR assays
Immunology: cytokeratin +, cytokeratin-7 +, cytokeratin-8 +, cytokeratin-17 +, cytokeratin-18 +, cytokeratin-19 +, desmin -, endothel -, EpCAM +, GFAP -, neurofilament -, vimentin +
Storage and Shipping
Frozen with 70% medium, 20% FBS, 10% DMSO at about 2 x 10^6 cells/ampoule; ship in dry ice; store in liquid nitrogen
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