The cells accumulate iodide intracellularly. Absolute requirement for 0.1 to 0.5% calf serum in addition to insulin, hydrocortisone, thyrotropin, transferrin, somatostatin and glycyl-1-histidyl-lysine acetate. These cells tend to grow on top of one another, forming three dimensional structures rather than a monolayer.
Coons modification of Ham's F12 medium supplemented with 2 mM L-glutamine, 0.01 I.U./ml TSH, 10 µg/ml insulin, 10 nM hydrocortisone, 5 µg/ml transferrin, 10 ng/ml somatostatin, 10 ng/ml glycyl-L-histidyl-L-lysine acetate and 5% fetal bovine serum.
Tests for mycoplasma, bacteria and fungi were negative
Storage and Shipping
liquid nitrogen vapor phase
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