The cells require TSH for growth; however, they should be placed in medium without TSH for studies involving responses to TSH. The cells tend to grow one above another, forming three dimensional structures rather than expanding into a monolayer. The species was confirmed by Real-Time PCR.
Coons modified Ham's F12 medium supplemented with L-glutamine, 10 µg/ml insulin, 10 nM hydrocortisone, 5 µg/ml transferrin, 10 ng/ml somatostatin, 10 ng/ml glycyl-L- histidyl-L-lysine acetate, 10 mUnits/ml TSH and 5% bovine calf serum.
Tests for mycoplasma, bacteria and fungi were negative
Storage and Shipping
liquid nitrogen vapor phase
If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the Pub Med ID of your paper to get a coupon.