Established from the malignant ascitic effusion from a woman with metastatic breast carcinoma; described as being estrogen receptor-negative and progesterone receptor-positive
Human hypotriploid karyotype with 4% polyploidy - 62(55-64)<3n>XXX, +1, -2, -5, -5, +6, -8, -9, -10, -13, -13, -14, -15, -16, -17, -18, -20, -22, +6-8mar, add(X)(p11), der(X)t(X;9)(q21;q11), add(1)(p1?1), der(1)del(1)(p36)dup(1)(q22q32), del(1)(q11), der(
adherent epitheloid cells growing in monolayers
Mycoplasma: contamination was eliminated with Ciprobay (ciprofloxacin), then negative in DAPI, microbiological culture, RNA hybridization, PCR assays
Immunology: cytokeratin +, cytokeratin-7 +, cytokeratin-8 +, cytokeratin-17, cytokeratin-18 +, cytokerati
Storage and Shipping
Frozen with 70% medium, 20% FBS, 10% DMSO at about 2 x 10^6 cells/ampoule; ship in dry ice; store in liquid nitrogen
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