DNA profiling studies revealed that STR patterns of the endothelial line ECV-304 and the human bladder line T24 were very similar, suggesting that ECV-304 was a derivative of T24. Furthermore, ATCC karyotypes of the two lines show two shared-marker chromosomes. Combined, these results show that ECV-304 is indeed a derivative of T24, a line that was developed years earlier. It is important to emphasize that all stocks of ECV show similar properties.
Homo sapiens (human)
urinary bladder; carcinoma
adherent growth as cobblestone monolayers
STR DNA Profile
Penta E: 7,10
Penta D: 11,15
Human hypertriploid karyotype with 6.7% polyploidy - 78(75-81)<3n>XX, -X, +1, -4, +5, +5, +5, +7, -8, -9, -10, +14, -15, -15, +17, +18, +20, +20, +20, +21, +22, +4-6mar - add(1)(p32)x1-2, i(1p), der(2q;5q), der(3)t(3;11)(p25;q13), i(5p)x2, inv(7)(p14q31)
Fluorescence (DAPI) test: negative; Mycoplasma specific PCR: negative; Bacteria
specific PCR: negative
Storage and Shipping
Frozen with 70% medium, 20% FBS, 10% DMSO at about 1 x 10^6 cells/ampoule; ship in dry ice; store in liquid nitrogen
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