The EB1 cell line was isolated by M.A. Epstein and Y.M. Barr in 1963 from biopsy fragments and cell clumps of a lymphoma. These were seeded into Insulin bottles in Eagles basal medium with 10% human serum and fed by medium addition. The cells grew as free-floating singel individuals or doublets with a mean doubling time of about 48 hrs and were identified as altered lymphoblasts on the basis of growth and uniform morphology.
Homo sapiens ,human
STR DNA Profile
Amelogenin: X ,X
Penta E: 13,17
Penta D: 6.4,13
Chromosome Frequency Distribution 30 cells: 2n = 46 The cell line is aneuploid human female, with chromosome counts in the near dipoid-range. Normal chromosomes N8, N11 and N14 are monosomic, with the remainder of autosomes usually being paired. The X chromosome most often is trisomic. Four marker chromosomes are found. Two of these (markers M1 and M3) involve the reciprocal translocation between chromosomes N8 and N14 associated with most Burkitts lymphoma cell lines first described by L. Zech et al., Int.J.Cancer 17: 47, 1976, with the further elaboration o fhte myc and Ig gene role by the reports of P. Leder, R. Taub and C.M. Croce (Science Washington DC) 222: 765, 1983; PNAS USA 79: 7824 and 7837, 1982.
round, single or aggregated cells in suspension
Mycoplasma: negative in DAPI, microbiological culture, RNA hybridization assays
Immunology: CD3 -, CD10 +, CD13 -, CD19 +, CD20 +, CD34 -, CD37 +, cyCD79a +, CD80 +, CD138 -, HLA-DR +, sm/cyIgG -, sm/cyIgM -, sm/cykappa -, sm/cylambda -
Viruses: ELISA: re
Storage and Shipping
Frozen with 70% medium, 20% FBS, 10% DMSO at 5 x 10^6 cells/ampoule; ship in dry ice; store in liquid nitrogen
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