DSL-6B/C2 is a pancreatic ductal cell line derived from the DSL-6 transplantable acinar cell carcinoma. The DSL-6 tumor was established in 1986 from a primary acinar cell carcinoma of the pancreas which developed in a male Lewis rat(DSL-101-79) that was given azaserine intraperitoneally. The cultured DSL-6B/C2 tumor cells initially produced amylase, but production of exocrine enzymes ceased after 1 to 2 weeks in culture. The cell line also lost structural and immunohistochemical acinar cell markers while acquiring duct cell markers during culture and regrafting.The species was confirmed by Real-Time PCR.
Waymouth medium supplemented with L-glutamine and 10% fetal bovine serum.
Tests for mycoplasma, bacteria and fungi were negative
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liquid nitrogen vapor phase
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