The DPSCs have been isolated from Human Pulp Tissue acc. to the procedure described in Ref. 1 and 2. The adherent cellular fraction of the enzymatically digested tissue was cultivated until about 70-80% confluence. After subculturing two times, the cells were cryopreserved in Passage 2.
Homo sapiens (human)
DMEM:Ham's F12, supplemented with L-glutamine, 15mM Hepes, and 5% fetal bovine serum.
Tests for mycoplasma (PCR, DAPI), bacteria and fungi (fluorescence) were negative.
If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the Pub Med ID of your paper to get a coupon.