Established from the tumor of a 56-year-old Caucasian man with pancreas adenocarcinoma in 1975. The cells produce high levels of MUC-1 mucin mRNA, low levels of MUC-2 mRNA but do not express the MUC-3 gene.
Homo sapiens (human)
STR DNA Profile
Penta E: 11
Penta D: 13,15
Human hypertriploid karyotype with 6% polyploidy - 68-73<3n>XX, -Y, +1, +1, +4, +5, +7, +11, -12, -14, -17, -18, -22, +3mar - del(1)(p11p33)x2, add(11)(p11), der(11)t(?8;11)(q12;p11)x2, i(14q)x2, add(21)(p11)x2, 1-2DM(24%) - sideline with +1, i(1q)x2, del
Fluorescence (DAPI) test: negative; Mycoplasma specific PCR: negative; Bacteria
specific PCR: negative
Storage and Shipping
Frozen with 70% medium, 20% FBS, 10% DMSO at about 1-2 x 10^6 cells/ampoule; ship in dry ice; store in liquid nitrogen
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