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COLO-824

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Cat.No.
CSC-C0259
Description
Established from the pleural fluid of a 52-year-old man with breast carcinoma in 1991. The cells do not tolerate DMSO; upon thawing, DMSO has to be removed by centrifugation.
Organism
Homo sapiens (human)
STR DNA Profile
Amelogenin: X,X
CSF1PO: 10,12
D16S539: 13
D13S317: 11,12,13
D5S818: 12
D7S820: 8,11
THO1: 7,9
TPOX: 6,11
vWA: 16
D3S1358: 16,17
D21S11: 28
D18S51: 15,19
Penta E: 7
Penta D: 5,10
D8S1179: 12,14
FGA: 22
Karyotype
Human highly rearranged hyperdiploid karyotype with 11% polyploidy - 51(48-52)<2n>X, -Y, +1, -2, -6, +7, -10, -13, -15, -19, -21, -22, +12mar, del(1)(p12), der(1)t(1;?)(p12;?), der(6)t(1;6)(q25;q12), der(7)t(7;?)(q32/34;?), der(8)t(8;?)(q24;?), der(12).
Source
Metastatis of a female breast cancer patient. (pleural effusion)
Morphology
Epithelial
Recommended Medium
RPMI-1640 medium supplemented with 2 mM L-glutamine and 10% fetal bovine serum.
Quality Control
Fluorescence (DAPI) test: negative; Mycoplasma specific PCR: negative; Bacteria
specific PCR: negative
Storage and Shipping
Frozen with 70% medium, 20% FBS, 10% DMSO at about 5 x 10^6 cells/ampoule; ship in dry ice; store in liquid nitrogen
Citation Guidance
If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the Pub Med ID of your paper to get a coupon.
CSC-6288W
CSC-6289W
CSC-6290W
CSC-6291W
CSC-6292W
CSC-6293W
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