This COLO 205 cell line was established from the ascites fluid obtained from a 70-year-old male patient with adenocarcinoma of the colon (Dukes Classification Grade D). The patient had been treated with 5-fluorouracil for 4-6 weeks before removal of the fluid specimen. The cells are CSAp negative (CSAp-) and positive for keratin by immunoperoxidase staining. COLO 205 cells express a 36,000 Dalton cell surface glycoprotein related to the GA733-2 tumor associated antigen. This cell line can produce carcinoembryonic antigen (CEA) 1.5 to 4.1 ng/10 exp6 cells/10 days, keratin and interleukin 10 (IL-10, interleukin-10).
Homo sapiens, human
STR DNA Profile
Penta E: 13,15
Penta D: 9,11
The stemline chromosome number is hypertriploid and 10-11 marker chromosomes (M1, M2, M4, M5, M6 and 5 others) were common in monosomic, disomic and trisomic condition although most of these markers were monosomic.
Fluorescence (DAPI) test: negative; Mycoplasma specific PCR: negative; Bacteria
specific PCR: negative
Storage and Shipping
liquid nitrogen vapor phase
Colorectal adenocarcinoma; Dukes' type D
This cell line can be used as a suitable transfection host.
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