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COLO-205

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Cat.No.
CSC-C8203L
Description
This COLO 205 cell line was established from the ascites fluid obtained from a 70-year-old male patient with adenocarcinoma of the colon (Dukes Classification Grade D). The patient had been treated with 5-fluorouracil for 4-6 weeks before removal of the fluid specimen. The cells are CSAp negative (CSAp-) and positive for keratin by immunoperoxidase staining. COLO 205 cells express a 36,000 Dalton cell surface glycoprotein related to the GA733-2 tumor associated antigen. This cell line can produce carcinoembryonic antigen (CEA) 1.5 to 4.1 ng/10 exp6 cells/10 days, keratin and interleukin 10 (IL-10, interleukin-10).
Tissue of Origin
Colon
Organism
Homo sapiens (human)
Culture Properties
suspension
Morphology
Epithelial
STR DNA Profile
Amelogenin: X,X
CSF1PO: 11,12
D13S317: 10,12
D16S539: 12,13
D5S818: 10,13
D7S820: 9,10
THO1: 8,9
TPOX: 11
vWA: 15
D3S1358: 16
D21S11: 30.2,33.2
D18S51: 18
Penta E: 13,15
Penta D: 9,11
D8S1179: 9,14
FGA: 21,23
Karyotype
The stemline chromosome number is hypertriploid and 10-11 marker chromosomes (M1, M2, M4, M5, M6 and 5 others) were common in monosomic, disomic and trisomic condition although most of these markers were monosomic.
Disease
Colorectal adenocarcinoma; Dukes' type D
Applications
This cell line can be used as a suitable transfection host.
Quality Control
Fluorescence (DAPI) test: negative; Mycoplasma specific PCR: negative; Bacteria
specific PCR: negative
Storage and Shipping
liquid nitrogen vapor phase
Citation Guidance
If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the Pub Med ID of your paper to get a coupon.
Customer Support & Price Inquiry
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