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CCRF-CEM

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Cat.No.
CSC-C8218L
Description
This cell line was a T lymphoblastoid line obtained from the peripheral blood of a 4 year old Caucasian female with acute lymphoblastoid leukaemia. These may be grown to a high density in a spinner type suspension culture. Cells are reported to be free of virus particles as determined by electron microscopy.
Tumorigenecity: yes, in syrian hamsters
Isoenzyme: G6PD, B
Histopathology: leukemia, acute lymphoblastic
Tissue of Origin
Blood
Organism
Homo sapiens, human
STR DNA Profile
Amelogenin: X
CSF1PO: 10,11
D13S317: 11,12
D16S539: 10,13
D5S818: 12,13
D7S820: 9,13
THO1: 6,7
TPOX: 8
vWA: 17,19
Karyotype
modal number = 47; range = 41 to 95.
Fifty karyotypes showed no consistent loss or gain of particular chromosomes. Twenty-eight percent of the cells with 45 chromosomes were C-; 53% of all cells had an extra D and 35% had an extra F. Only N1 and N18 were not affected by gain or loss. No marker chromosomes noted.
Source
Blood
Culture Properties
Suspension
Morphology
lymphoblast
Recommended Medium
Quality Control
Tests for mycoplasma, bacteria and fungi were negative
Storage and Shipping
liquid nitrogen vapor phase
Application
The CCRF-CEM cell line can be used as a suitable transfection host.
Citation Guidance
If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the Pub Med ID of your paper to get a coupon.
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