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CADO-ES1

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Cat.No.
CSC-C2561
Description
Established from the malignant pleural effusion from a 19-year-old Japanese woman diagnosed with right-buttock Ewings sarcoma with multiple lung metastases; heterotransplantable; capable of anchorage-independent growth; neural differentiation in response to cAMP.
Organism
Homo sapiens (human)
STR DNA Profile
Amelogenin: X,X
CSF1PO: 11,12
D13S317: 10,13
D16S539: 9,11
D5S818: 11,12
D7S820: 11,13
THO1: 6,9
TPOX: 8,11
vWA: 14,18
D3S1358: 16,18
D21S11: 31,32.2
D18S51: 15,20
Penta E: 12,19
Penta D: 13
D8S1179: 12,15
FGA: 21,22
Karyotype
Human hyperdiploid karyotype with 8% polyploidy - 47(44-48)<2n>XX, +8, dup(1)(q32q44), i(8q), add(18)(p11.32)-t(11;22) absent-corresponds to published karyotype-absence of EWS-FLI1 fusion transcripts confirmed by RT-PCR.
Source
Ewing’s sarcoma
Morphology
Small round tumor cells
Recommended Medium
Quality Control
Mycoplasma: negative in DAPI, microbiological culture, RNA hybridization, PCR assays
Immunology: cytokeratin -, cytokeratin-7 -, cytokeratin-8 -, cytokeratin-17 -, cytokeratin-18 -, desmin -, endothel -, GFAP -, HMB-45 -, neurofilament +, vimentin +
Storage and Shipping
Frozen with 70% medium, 20% FBS, 10% DMSO at about 1.5 x 10^6 cells/ampoule; ship in dry ice; store in liquid nitrogen
Citation Guidance
If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the Pub Med ID of your paper to get a coupon.
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