The BT-549 line was isolated in 1978 by W.G. Coutinho and E.Y. Lasfargues. Source tissue consisted of a papillary, invasive ductal tumor which had metastasized to 3 of 7 regional lymph nodes. The established population was polymorphic with epithelial like components and multinucleated giant cells. A mucin-like material was secreted into the medium.
Homo sapiens, human
STR DNA Profile
Amelogenin: X, X
CSF1PO: 10, 12
D7S820: 9, 10
Penta E: 14
Penta D: 13
D8S1179: 14, 16
modal number = 78; range = 73 to 80.
The cell line is aneuploid human female, with chromosome counts in the hypertriploid range. The X chromosomes are all abnormal. Normal chromosomes N10 and N13 are clearly under-represented, with chromosomes N2, N12, and N17 also tending to be. Chromosome N8 is over-represented with respect to the copy number of other normal chromosomes, and chromosomes N5 and N18 also usually have more copies than most chromosomes. Four marker chromosomes are found: iso(13q), 10q+, del(X)(q22:), del(11)(p11:).
Fluorescence (DAPI) test: negative; Mycoplasma specific PCR: negative; Bacteria
specific PCR: negative
Storage and Shipping
Liquid nitrogen vapor phase
Invasive ductal carcinoma
This cell line can be used as a suitable transfection host.
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